Phenolic Constituents of <i>Elsholtzia blanda</i> Benth

Objectives: Elsholtzia blanda Benth. is a Vietnamese medicinal plant, used to treat inflammation, cough, and flu. Therefore, this current study focused on the isolation of active ingredients from plant their NO production inhibitory activity. Methods: E. aerial parts were dried, minced, ultrasonically extracted with methanol further separated by various chromatographic methods. The chemical structures isolates revealed nuclear magnetic resonance, high resolution lectrospray ionization mass spectrometry, circular dichroism spectra. nitric oxide inhibition effects evaluated in lipopolysaccharide-activated RAW264.7 cells. Results: Ten phenolic components [1 new (1) 9 known (2-10)] isolated determined. Compound 5 (innovanoside, IC 50 = 19.23 ± 1.43 µM) 6 (maltol- O- β-D-glucopyranoside, 21.73 1.27 showed highest activity, 2-4 moderate (IC 47.36 ∼ 57.29 µM). other compounds inactive values &gt;100 µM. Conclusions: One previously undescribed have been screened for Compounds 2-6 significant 19.23∼57.29